In this research, we seek to understand the concept of entrepreneurial opportunity and explain the opportunity development process in the Individual Opportunity (IO) Nexus Framework. We applied the principle of the science of artificial to study the opportunity development process. From this principle, opportunity is existed as an objective phenomenon and can be connected and transformed into business ideas. The opportunity development process was designed by using the design process and the 2x2 factorial designs and a procedure. The instruments and procedure were developed and tested to ensure validity and effectiveness. The results suggested the instruments are valid and the designed process is effective.