The explosive growth in technology usage has put growing pressure on organizations to serve customers electronically. Healthcare service, as one of data intensive industries, tries to serve their patients with excellent service in the timely manner when the data volume is growing faster than organizational infrastructure development. Because the hospital information system called Electronic Medical Record (EMR) is a powerful tool, and provides a multitude of benefits, many hospitals are today considering accepting, and adapting this technology to provide medical information and healthcare services in a better way. This study will extend applicability of the Technology Acceptance Model (TAM) to test user acceptance of EMR in the hospital context in Thailand. The study aims to investigate EMR characteristics which affect Electronic Medical Record (EMR) acceptance in the healthcare profession’s belief perspective in hospital. This EMR acceptance measurement will help the organization forecast how healthcare professions’ perception of EMR usage will affect and support the organizational development. The result can be used as a guideline for EMR implementation that may lead to a successful EMR imitative in Thailand.